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KMID : 0980720020210020191
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Keimyung Medical Journal
2002 Volume.21 No. 2 p.191 ~ p.201
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Induction of Antiviral Antibody using HBV Gene and HBV mRNA Pulsed Antigen Presenting Cell
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Park Jong-Wook
Kwon Taeg-Kyu
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Abstract
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In order to develop effective vaccine to recover incomplete immunity in patients with chronic hepatitis B virus infection, we produced various types of vaccine for Hepatitis B virus core (HBc) antigen and compared their effects on anti-HBc antibody response. Intramuscular injection of HBc DNA vaccine (pc -HBc) and intraperitoneal injection of HBc-transfected Renca and EL-4 effectively induced anti-HBc antibody response in mouse. Lipopolysaccharide (LPS) induced gene expressions of IP-10, GM-CSF, and IL-12 in dendritic cell (DC). However, LPS-stimulated DC or peritoneal macrophage, that were trasnsfected with HBc mRNA, on HBc mRNA vaccine itself induced lower anti-HBc antibody response in mouse compared to pc-HBc vaccine. These results suggest HBc DNA vaccine is more simple and effective for the induction of anti-HBc antibody response than HBc mRNA or mRNA-transfected cell vaccine.
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KEYWORD
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Core antigen, Hepatitis B virus, Vaccine
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